~1 spots leftby Jun 2025

CAR T Cell Therapy for Pediatric Solid Cancers

Recruiting in Palo Alto (17 mi)
Age: < 65
Sex: Any
Travel: May Be Covered
Time Reimbursement: Varies
Trial Phase: Phase 1
Recruiting
Sponsor: Seattle Children's Hospital
Must not be taking: Anti-cancer agents, Chemotherapy
Disqualifiers: Active malignancy, CNS pathology, Severe infection, others
No Placebo Group
Approved in 1 Jurisdiction

Trial Summary

What is the purpose of this trial?

This is a phase I, open-label, non-randomized study that will enroll pediatric and young adult research participants with relapsed or refractory non-CNS solid tumors to evaluate the safety, feasibility, and efficacy of administering T cell products derived from the research participant's blood that have been genetically modified to express a EGFR-specific receptor (chimeric antigen receptor, or CAR) that will target and kill solid tumors that express EGFR and the selection-suicide marker EGFRt. EGFRt is a protein incorporated into the cell with our EGFR receptor which is used to identify the modified T cells and can be used as a tag that allows for elimination of the modified T cells if needed. On Arm A of the study, research participants will receive EGFR-specific CAR T cells only. On Arm B of the study, research participants will receive CAR T cells directed at EGFR and CD19, a marker on the surface of B lymphocytes, following the hypothesis that CD19+ B cells serving in their normal role as antigen presenting cells to T cells will promote the expansion and persistence of the CAR T cells. The CD19 receptor harbors a different selection-suicide marker, HERtG. The primary objectives of the study will be to determine the feasibility of manufacturing the cell products, the safety of the T cell product infusion, to determine the maximum tolerated dose of the CAR T cells products, to describe the full toxicity profile of each product, and determine the persistence of the modified cell in the subject's body on each arm. Subjects will receive a single dose of T cells comprised of two different subtypes of T cells (CD4 and CD8 T cells) felt to benefit one another once administered to the research participants for improved potential therapeutic effect. The secondary objectives of this protocol are to study the number of modified cells in the patients and the duration they continue to be at detectable levels. The investigators will also quantitate anti-tumor efficacy on each arm. Subjects who experience significant and potentially life-threatening toxicities (other than clinically manageable toxicities related to T cells working, called cytokine release syndrome) will receive infusions of cetuximab (an antibody commercially available that targets EGFRt) or trastuzumab (an antibody commercially available that targets HER2tG) to assess the ability of the EGFRt on the T cells to be an effective suicide mechanism for the elimination of the transferred T cell products.

Will I have to stop taking my current medications?

The trial requires that you stop taking any anti-cancer agents or chemotherapy before enrolling. If you have been on certain therapies, there are specific waiting periods before you can join the trial, such as 7 days after the last chemotherapy or biologic therapy, and 30 days after the last dose of anti-tumor antibody therapy.

What data supports the effectiveness of the treatment EGFR806 CAR T Cell Immunotherapy for pediatric solid cancers?

Research shows that EGFR806 CAR T cells can effectively target and destroy tumor cells expressing a specific form of the EGFR protein, as demonstrated in glioblastoma and non-small cell lung cancer studies. These cells have shown the ability to selectively attack cancer cells without harming normal cells, indicating potential effectiveness in treating solid tumors.12345

Is CAR T Cell Therapy safe for treating pediatric solid cancers?

Research on EGFR-targeted CAR T cell therapy, which is similar to the therapy being studied, shows that it was well-tolerated without severe toxicity in patients with lung cancer. This suggests that the therapy may be generally safe in humans, although serious adverse events have been noted in other CAR T cell therapies, so monitoring is important.12678

How is EGFR806 CAR T Cell Immunotherapy different from other treatments for pediatric solid cancers?

EGFR806 CAR T Cell Immunotherapy is unique because it uses specially engineered T cells to target a specific part of the EGFR protein found on tumor cells, which helps it selectively attack cancer cells without harming normal cells. This approach is different from traditional treatments like chemotherapy and radiation, which can affect both healthy and cancerous cells.1291011

Research Team

KA

Katie Albert, MD

Principal Investigator

Seattle Children's Hospital

Eligibility Criteria

This trial is for children and young adults aged 1-30 with certain non-CNS solid tumors that are recurrent or resistant to treatment. Participants must have a life expectancy of at least 8 weeks, be able to undergo apheresis (a procedure to collect immune cells), and not have had recent myeloablative therapy unless they've recovered. They should not be receiving other cancer treatments or have active infections, CNS pathology, GVHD, primary immunodeficiency, or be pregnant.

Inclusion Criteria

I am between 15 and 30 years old.
It's been over 6 weeks since my last intense therapy and stem cell transplant.
Patients of childbearing potential must agree to use highly effective contraception
See 18 more

Exclusion Criteria

Pregnant or breastfeeding
Unwilling to provide consent/assent for participation in the study and 15 year follow up period
I have a current brain or spinal cord condition.
See 7 more

Trial Timeline

Screening

Participants are screened for eligibility to participate in the trial

2-4 weeks

Treatment

Participants receive a single dose of genetically modified T cells (CD4 and CD8) targeting EGFR and potentially CD19

4 weeks
1 visit (in-person)

Follow-up

Participants are monitored for safety, efficacy, and persistence of CAR T cells in the blood and bone marrow

12 weeks
Multiple visits (in-person)

Long-term follow-up

Participants are monitored for long-term safety and efficacy, including anti-tumor responses and survival characteristics

84 days

Treatment Details

Interventions

  • EGFR806 CAR T Cell Immunotherapy (CAR T-cell Therapy)
Trial OverviewThe study tests genetically modified T cells designed to target EGFR on tumors in two groups: one receives CAR T cells targeting only EGFR; the other targets both EGFR and CD19. The goal is to see if these T cells are safe, can be made successfully from participants' blood, what dose is tolerated best without severe side effects (except manageable ones like cytokine release syndrome), how long they last in the body, and their effectiveness against tumors.
Participant Groups
2Treatment groups
Experimental Treatment
Group I: EGFR806CAR(2G)-EGFRt and CD19CAR(2G)-T2A-HER2tGExperimental Treatment1 Intervention
Autologous CD4+ and CD8+ T cells that have been genetically modified to express the EGFR806CAR(2G)-EGFRt and CD19CAR(2G)-T2A-HER2tG
Group II: EGFR 806CAR(2G) -EGFRtExperimental Treatment1 Intervention
Autologous CD4+ and CD8+ T cells that have been genetically modified to express the EGFR 806CAR(2G) -EGFRt

Find a Clinic Near You

Research Locations NearbySelect from list below to view details:
Seattle Children's HospitalSeattle, WA
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Who Is Running the Clinical Trial?

Seattle Children's Hospital

Lead Sponsor

Trials
319
Patients Recruited
5,232,000+

Findings from Research

Chimeric antigen receptor-modified T cells for the immunotherapy of patients with EGFR-expressing advanced relapsed/refractory non-small cell lung cancer.Feng, K., Guo, Y., Dai, H., et al.[2022]
EGFR806-CAR T cells selectively target a tumor-restricted EGFR epitope in glioblastoma.Ravanpay, AC., Gust, J., Johnson, AJ., et al.[2020]
ErbB-targeted CAR T-cell immunotherapy of cancer.Whilding, LM., Maher, J.[2022]
Chimeric antigen receptor (CAR) T cell therapy has shown robust efficacy in treating pediatric B cell acute lymphocytic leukemia (ALL), providing a potential cure for children with this previously lethal disease.
Pediatric cancers are particularly suitable for immune-based therapies due to their unique developmental origins and the expression of specific cell surface molecules, although challenges remain in developing effective immunotherapeutics for these conditions.
Immune-Based Approaches for the Treatment of Pediatric Malignancies.Bosse, KR., Majzner, RG., Mackall, CL., et al.[2021]
A phase I clinical trial with monoclonal antibody ch806 targeting transitional state and mutant epidermal growth factor receptors.Scott, AM., Lee, FT., Tebbutt, N., et al.[2022]
A phase 1 study of ABT-806 in subjects with advanced solid tumors.Cleary, JM., Reardon, DA., Azad, N., et al.[2018]
A Fusion Receptor as a Safety Switch, Detection, and Purification Biomarker for Adoptive Transferred T Cells.Wu, X., Shi, B., Zhang, J., et al.[2018]
Serious adverse events and coping strategies of CAR-T cells in the treatment of malignant tumors.Chen, X., Li, P., Tian, B., et al.[2023]
CAR-T cells for pediatric brain tumors: Present and future.Leruste, A., Beccaria, K., Doz, F.[2021]
Advances in CAR T cell immunotherapy for paediatric brain tumours.Rao, P., Furst, L., Meyran, D., et al.[2022]
Chimeric Antigen Receptor T cell Therapy and the Immunosuppressive Tumor Microenvironment in Pediatric Sarcoma.Terry, RL., Meyran, D., Fleuren, EDG., et al.[2021]

References

Chimeric antigen receptor-modified T cells for the immunotherapy of patients with EGFR-expressing advanced relapsed/refractory non-small cell lung cancer. [2022]
EGFR806-CAR T cells selectively target a tumor-restricted EGFR epitope in glioblastoma. [2020]
ErbB-targeted CAR T-cell immunotherapy of cancer. [2022]
Immune-Based Approaches for the Treatment of Pediatric Malignancies. [2021]
A phase I clinical trial with monoclonal antibody ch806 targeting transitional state and mutant epidermal growth factor receptors. [2022]
A phase 1 study of ABT-806 in subjects with advanced solid tumors. [2018]
A Fusion Receptor as a Safety Switch, Detection, and Purification Biomarker for Adoptive Transferred T Cells. [2018]
Serious adverse events and coping strategies of CAR-T cells in the treatment of malignant tumors. [2023]
CAR-T cells for pediatric brain tumors: Present and future. [2021]
Advances in CAR T cell immunotherapy for paediatric brain tumours. [2022]
Chimeric Antigen Receptor T cell Therapy and the Immunosuppressive Tumor Microenvironment in Pediatric Sarcoma. [2021]